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Study of Retention Mechanism and Development of Hilic Methods pdf

Study of Retention Mechanism and Development of Hilic Methods pdf

Study of Retention Mechanism and Development of Hilic Methods by Dimitrovska Aneta

Study of Retention Mechanism and Development of Hilic Methods
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Author: Dimitrovska Aneta
Page Count: 92 pages
Published Date: 29 Nov 2013
Publisher: LAP Lambert Academic Publishing
Publication Country: United States
Language: English
Type: Pdf
ISBN: 9783659495304
File size: 48 Mb
File Name: Study.of.Retention.Mechanism.and.Development.of.Hilic.Methods.pdf
Download Link: Study of Retention Mechanism and Development of Hilic Methods
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In the last twenty years hydrophilic interaction liquid chromatography (HILIC) has emerged as a new type of liquid chromatography for separation of polar compounds. In HILIC, a multimodal retention mechanism is present so it is very difficult to predict the impact of chromatographic parameters on the retention and separation of the compounds. Furthermore, the relationship between the pH of the mobile phase, the degree of ionization of the compounds, the surface of the stationary phase and the absorbed water layer is very complex, which significantly complicates the HILIC method development. In this book we experimentally determinate the contribution of partition, adsorption and ion exchange mechanism to the retention of several polar compounds on bare silica column. Also fast, simple and easily applicable HILIC methods for determination of polar compounds in different dosage forms were established using the univariate and design of experiments approach. The book could serve as a guide for the HILIC method development and optimization, and should be useful for practitioners working in analytical method development laboratories.

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